Cell culture protocol for recording from QBM Cell Science - E14/15 Embryonic C57 Mouse Cortical Neurons on Maestro MEA systems.
Abstract:
Axion BioSystems multi-well and single-well microelectrode arrays (MEAs) are ideally suited for investigation of electroactive cells and tissue. The MEA wells are organized in an ANSI-SBS compliant format, compatible with traditional plate readers and automated instrumentation. Within each well, multiple substrate-embedded microelectrodes are each capable of monitoring the activity of numerous individual cells. The arrangement of these electrodes into a grid extends the recording range across a 1.43x1.43 mm (12-well), a 1.05x1.05 mm (48-well), or a 0.7x0.6 mm (96-well) area, providing concurrent access to both single-cell and network-level activity. Axion’s Integrated Studio (AxIS) software simplifies the process of performing MEA cell culture experiments. Our easy to use software provides complete access to critical information and total control of experimental parameters. AxIS allows concurrent monitoring of channel recordings, digital and analog filter adjustments, electrode assignment, and stimulus waveform design, all within the same application in an easy to use modular layout. This user guide will aid you in growing your E14,15 embryonic C57 mouse cortical neuron cultures on the microelectrode array (MEA). Neurons cultured using this protocol should show spike activity detectable in AxIS software by day 10 in vitro.
